A STUDY OF THE PRE-TREATMENT TEMPERATURE OF LIGNOCELLULOSIC BUTANOLS AN ALTERNATIVE FUEL (BIOFUEL) PRODUCED FROM BAMBOO USING CLOSTRIDIUM ACETOBUTYLICUM
Abstract:
The demand for sustainable and renewable energy sources has grown significantly in recent years. Biofuels, particularly lignocellulosic-based biofuels, have emerged as a promising alternative to fossil fuels. This study focuses on the production of butanols, specifically isobutanol and n-butanol, from bamboo biomass using the bacterium Clostridium acetobutylicum as a biocatalyst.
The aim of this research is to investigate the influence of pre-treatment temperature on the production efficiency of lignocellulosic butanols. Bamboo, a fast-growing and abundant lignocellulosic feedstock, was selected due to its high cellulose and hemicellulose content. The pre-treatment step is crucial as it enhances the accessibility of cellulose and hemicellulose to enzymatic hydrolysis, leading to higher sugar yields.
In this study, bamboo biomass was subjected to pre-treatment at different temperatures ranging from X°C to Y°C. The pre-treated biomass was then enzymatically hydrolyzed to release fermentable sugars, which were subsequently utilized by Clostridium acetobutylicum for butanol production through a two-step fermentation process.
The effects of pre-treatment temperature on sugar release, fermentation efficiency, and butanol yield were evaluated. Analytical techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) were employed to quantify sugar concentrations and determine the composition of the fermentation products.
Preliminary results indicate that the pre-treatment temperature significantly affects the efficiency of lignocellulosic butanol production. Higher pre-treatment temperatures exhibited improved sugar release, resulting in enhanced fermentation performance and higher butanol yields. However, excessively high temperatures may lead to the degradation of sugars and the formation of inhibitory compounds, negatively impacting the overall process.
Optimizing the pre-treatment temperature is crucial for maximizing the production of lignocellulosic butanols from bamboo biomass. This study provides valuable insights into the potential of bamboo as a renewable feedstock for biofuel production, while also highlighting the importance of optimizing pre-treatment conditions for efficient conversion of lignocellulosic biomass into valuable biofuels.
Keywords: Lignocellulosic butanols, biofuel, bamboo, Clostridium acetobutylicum, pre-treatment temperature, enzymatic hydrolysis, fermentation efficiency.
A STUDY OF THE PRE-TREATMENT TEMPERATURE OF LIGNOCELLULOSIC BUTANOLS AN ALTERNATIVE FUEL (BIOFUEL) PRODUCED FROM BAMBOO USING CLOSTRIDIUM ACETOBUTYLICUM, GET MORE MATERIALS SCIENCE AND ENGINEERING