Utilizing Saccharomyces cerevisiae CBS 1171 and Lactobacillus delbrueckii MN945906 for Bioethanol Production from Selected Agro Waste.
Abstract:
This study focuses on the production of bioethanol from agro waste, specifically banana and cassava peels. To achieve this, Saccharomyces cerevisiae strain CBS 1171 and Lactobacillus delbrueckii strain MN945906 were employed. The proximate analysis of the waste indicated a carbohydrate content of 67.85±0.23%, nitrogen at 0.21%, potassium at 64.7%, and phosphorus at 31.3%.
The waste was subjected to hydrolysis using Aspergillus niger strain MN945947 and Zymomonas mobilis strain MN945907, resulting in substrates of 20g, 30g, and 40g, which were left to ferment for 7 days. Following the fermentation, the extracts were filtered, and the resulting product was purified through distillation, with ethanol collected at 78oC.
The fermentation process involved different combinations of S. cerevisiae and L. delbrueckii. When both strains were used together for the fermentation of 40g substrate, the bioethanol yield was 21.94% for banana+cassava, 25.99% for cassava peel, and 10.32% for banana. Using S. cerevisiae alone for fermentation, the bioethanol yield was 22.98% for cassava peel, 9.36% for banana, and 21.08% for banana+cassava peel. When L. delbrueckii was used alone for fermentation, the bioethanol yield was 10.61% for cassava peel, 8.24% for banana, and 15.79% for banana+cassava peel.
Further investigation using 30g of substrate revealed a bioethanol yield of 20.92% for cassava peel, 9.56% for banana peel, and 20.86% for banana+cassava peel. At 20g of substrate, the yields were 14.14% for cassava peel, 8.76% for banana peel, and 13.21% for banana+cassava peels.
The average percentage weight and volume for banana+cassava were 13.83% (15.7g/cm3), for cassava peel were 12.26% (14.17g/cm3), and for banana peel were 6.8% (18.24g/cm3).
Based on the results, it is evident that the combination of S. cerevisiae and L. delbrueckii with banana+cassava peels as the substrate showed the most promising production of bioethanol.
Utilizing Saccharomyces cerevisiae CBS 1171 and Lactobacillus delbrueckii MN945906 for Bioethanol Production from Selected Agro Waste.