Isolation and Characterization of Vancomycin-Resistant Staphylococcus aureus from Wound Infections in Patients Visiting Designated Hospitals in Minna, Niger State, Nigeria.

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Isolation and Characterization of Vancomycin-Resistant Staphylococcus aureus from Wound Infections in Patients Visiting Designated Hospitals in Minna, Niger State, Nigeria.

ABSTRACT:

This research focused on determining the antibiotic sensitivity of Staphylococcus aureus strains isolated from wound samples of patients attending General Hospital (GH) and IBB Specialist Hospital in Minna, Niger State. A total of 200 wound samples were collected and examined for the presence of Staphylococcus aureus. The study also assessed the resistance patterns of these isolates to vancomycin using disc diffusion techniques.

Among the collected samples from both hospitals, 97 (48.50 %) tested positive for Staphylococcus aureus. Notably, GH Minna exhibited a higher occurrence of S. aureus with 76 cases (50.70 %) compared to IBB Specialist Minna, which had 21 cases (42.00 %). Additionally, it was observed that male patients showed a higher frequency of Staphylococcus aureus infection, with 65 cases (67.00 %), compared to females with 32 cases (33.00 %).

The study unveiled a concerning trend of high resistance of Staphylococcus aureus to vancomycin, as well as other antibiotics like Amoxicillin, Ampicillin, Oxacillin, and Erythromycin. However, resistance to Erythromycin was relatively low in this investigation. Molecular characterization identified several VRSA (Vancomycin-Resistant Staphylococcus aureus) isolates, specifically strains GSI17, GSI36, GSI93, GSI112, and GSI114.

Furthermore, the study detected the presence of vanA, vanB, and vanXY resistant genes in these isolates, with band sizes approximately 713 bp, 430 bp, and 550 bp, respectively. These findings indicate the active circulation of vanA, vanB, and vanXY resistant genes among Staphylococcus aureus strains in the study area. Such insights are crucial for understanding antibiotic resistance patterns and guiding effective treatment strategies.

Isolation and Characterization of Vancomycin-Resistant Staphylococcus aureus from Wound Infections in Patients Visiting Designated Hospitals in Minna, Niger State, Nigeria.

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