EXTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL

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RESEARCH PROJECT TOPIC ON EXTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL

ABSTRACT
Crysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus 0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50 and A. flavons  0.40.
The pharmacological screening confirmed the medical value of this plant oil and it established a good support for the sample in herbal medicine and as a base for the development of new drugs and phytomedicine.

TABLE OF CONTENT

Title Page———i

Certification——–ii

Dedication———iii

Acknowledgement——-iv

Abstract ———vi

Table of Content——–vii

Chapter One

1.0 Introduction ——-1

1.1 Statement of Problem——4

1.2 Purpose of the Study——5

1.3 Significance of Study——8

1.4 Limitation——–9

1.5 Scope of Study——-11

Chapter Two

2.0 Review of Related Literature —-12

2.6 Summary of Literature Review—- 19

Chapter Three

3.0 Research Methodology and Procedure—22

3.1 Population ——–22

3.2 Sample and Sampling Technique—-22

3.3 Validation of the Instrument —-23

3.4 Reliability of the Instrument —–23

3.5 Data Analysis——-23

Chapter Four

4.0 Presentation and Discussion of Result—24

4.1 Analysis and interpretaion of Data—25

4.2 Discussion of Results——38

Chapter Five

5.0 Summary, Conclusion and Recommendation  –40

5.1 Summary——–40

5.2 Conclusion——–41

5.3 Recommendation——42

References ———45

Appendix 1——–47

Appendix ———50

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